our protocols

Too much time is spent in science optimizing a protocol you read in a paper. On this page you'll find protocols for some of our niche experiments as well as some of our 'boutique' assays that we routinely use in our laboratory.

Importantly, we encourage you to email the lab if you have any questions or comments regarding these assays!

Generating in vitro exhausted CD8+ T cells

Tumor Immunology 101: Implantable models from cells to mice to cells

ATP quantitation analysis

Metabolic flux analysis with small T cell numbers

Phospho-FACS protocol (InstantFix)

2NBDG uptake in vivo

Single cell metabolic capacity by flow cytometry